Experts in eukaryotic genome sequencing
Obtain a draft genome of your study species with our eukaryotic genome sequencing service
Obtaining a draft genome of your protist, fungi, animal, or plant under study is of great utility in several research fields. For example, in comparative genomics, when we need to characterise regions up- and downstream the genes of interest. In RNA-seq, where we need an annotated reference genome for read mapping. Or in phylogenomics, in which novel genome regions including mitochondrial and chloroplast genomes can be sequenced.
Our lab and bioinformatics teams will work together to offer you the most suitable workflow to get the genome of your study species. Our expertise encompasses high-quality DNA extraction protocols, both short-read and long-read (e.g. Oxford Nanopore) sequencing, and assembly of difficult, large genomes.
Step 1
We isolate DNA from your samples. We have adapted different DNA isolation protocols, depending on the starting biological material.
Step 2
We prepare genomic libraries using the kits recommended by Illumina.
Step 3
We sequence the libraries using the Illumina NovaSeq technology. As a norm, we obtain 50 or 100 gigabases of raw data per library but we can adapt the output to the genome size of the species under study.
Step 4
We analyse the high-throughput sequencing data to obtain your annotated contigs.
What you receive
